- Institution/s: Universidad de Valladolid, Universitatea de Vest din Timişoara
- Project: Diccionario Terminológico de Genética (Eurogene)
- Identification number: 114
- Subdomains: Studies of chromatin, Studies of DNA, Studies of RNA, Techniques
- Author/s: Ana MUÑOZ GASCÓN, Bianca CONSTANTINESCU, Isaac MORENO RUIZ, María Eugenia HERRERAS
- Creation / modification date: 01-02-2014
- Term: uracil
- Gramatical category: s
- Definition: One of four chemical bases that are part of RNA. The other three bases are adenine (A), cytosine (C), and guanine (G). In DNA, the base thymine (T) is used in place of uracil.
- Context: We report that carry-over contamination can be controlled by the following two steps: (i) incorporating dUTP in all PCR products (by substituting dUPT for dTTP, or by incorporating uracil during synthesis of the oligodeoxyribonucleotide primers; and (ii) treating all subsequent fully preassembled starting reactions with uracil DNA glycosylase (UDG), followed by thermal inactivation of UDG. UDG cleaves the uracil base from the phosphodiester backbone of uracil-containing DNA, but has no effect on natural (i.e., thymine-containing) DNA.
- Expression: .
- Weighting mark: nor
- Sources:
- Term: http://www.futura-sciences.com/magazines/sante/infos/dico/d/genetique-arn-97/
- Definition: http://www.futura-sciences.com/magazines/sante/infos/dico/d/genetique-uracile-280/
- Context: http://www.futura-sciences.com/magazines/sante/infos/dico/d/genetique-arn-97/
- Expression: http://www.scienceinschool.org/2011/issue18/uracil/french
- Images:
http://es.wikipedia.org/wiki/Uracilo
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