- Institution/s: Universidad de Valladolid, Universitatea de Vest din Timişoara
- Project: Diccionario Terminológico de Genética (Eurogene)
- Identification number: 58
- Subdomains: Studies of chromatin, Studies of DNA, Studies of RNA, Techniques
- Author/s: Carmen CUÉLLAR LÁZARO, Isabel COMAS MARTÍNEZ, Laura SANJUÁN LANCHO, Lucia UDRESCU
- Creation / modification date: 02-03-2014
- Term: hybridisation
- Gramatical category: s
- Definition: Process of combining two complementary strands of DNA or RNA molecules and allowing them to form a single double-stranded nucleic acid through base pairing.
- Context: After transfer and appropriate treatment of the paper to destroy remaining diazo groups, specific RNA bands can be detected by hybridization with 32P-labeled DNA probes followed by autoradiography.
- Weighting mark: nor
- Sources:
- Term: EMERY, A.E.H. y MUELLER, R.F. (1992) Elements of Medical Genetics, Churchill Livingstone: New York. 8th Edition. (p. 114)
- Definition: - Biology online. Dictionary. [en línea]. Disponible en: [consulta: 30 abril 2013] - GENETICS HOME REFERENCE: Glossary [en línea]. EE.UU, última actualización 6 de junio de 2011. Disponible en: http://ghr.nlm.nih.gov/glossary [Consulta: 30 abril 20113]
- Context: ALWINE, J. C., KEMP, D. J., & STARK, G. R. (1977). Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes. Proceedings of the National Academy of Sciences, 74(12), 5350-5354. Recuperado el 30 de abril de 2013 de: http://www.pnas.org/content/74/12/5350.short
- Images:
http://ccrhawaii.org/index.php/nucleic-acid-techniques/23-nucleic-acid-hybridization-a-expression-analysis/23c-in-situ-hybridization-a-dna-microarrays/23c-content-tutorial
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