- Institution/s: Universidad de Valladolid
- Project: Diccionario Terminológico de Genética (Eurogene)
- Identification number: 17
- Subdomains: Studies of chromatin, Studies of DNA, Studies of RNA, Techniques
- Author/s: Marta VALDENEBRO ARENAS, Patricia ROJO DE DOMINGO, Regina CAVA GARCÍA, Rubén ORTIZ PAMPLIN
- Creation / modification date: 01-03-2014
- Term: pulsed field gel electrophoresis
- Gramatical category: s
- Definition: Technique of DNA analysis using electrophoretic methods to separate large DNA framents, up to 2 million base pairs in size, produced by digesting DNA with restriction enzymes with relatively long DNA recognition sequences which as a consequence cut the DNA relatively infrequently.
- Context: Use of pulsed field gel electrophoresis (PFGE), in which DNA fragments are subjected alternately to two approximately perpendicular fields, enables separation of DNA fragments of up to 2 000 000 base-pairs in size and with recent improvements in the technique up to 5–10 000 000 base-pairs in size.
- Expression: Fractionated by PFGE Isolated by PFGE
- Weighting mark: nor
- Sources:
- Term: PASSARGE, Eberhard (2008). Taschenatlas Humangenetik. Stuttgart: Georg Thieme Verlag, 371
- Definition: PASSARGE, Eberhard (2008). Taschenatlas Humangenetik. Stuttgart: Georg Thieme Verlag, 371
- Context: SEYFFERT, Willhelm (2003). Lehrbuch der Genetik. Heidelberg: Spektrum Akademischer Verlag, 1070.
- Images:
http://www.snv.jussieu.fr/bmedia/ATP/bioch6.htm
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